咨询服务电话:400 8717 699

简单实验流程,节约时间和成本

1小时3步组装具有编辑功能的Alt-R® RNP

多种方式高效导入Alt-R® RNP

提供针对不同模式生物的实验方法

Alt-R® RNP可在体外对DNA进行编辑


优化的gRNA —Alt-R® crRNA : tracrRNA复合体

crRNA: tracrRNA 优化长度显著改善编辑效率

特殊修饰crRNA:tracrRNA 增加核酸酶抗性降低免疫反应

多物种在线crRNA预设计数据库及检查器


“千挑万选”高保真Cas9酶 —Alt-R® S.P. HIFI Cas9

对目标位点较高的编辑效率

基因编辑脱靶效应显著降低

与gRNA复合体形成RNP,细胞毒性低,不易引起免疫反应

 

人类干细胞上的测试结果表明,与其他高保真Cas9酶比, IDT新高保真Cas9酶呈现出稳定的中靶编辑活性,脱靶率又极低。我们被IDT新高保真Cas9酶的表现所折服,已在使用它来开发基于基因编辑的疾病治疗方案。
--马修·波特斯(Matthew Porteus) 博士,干细胞移植专家,美国斯坦福大学

文献列表

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  • Tr?der SE, Eber LK, et al.?(2018)?An optimized electroporation approach for efficient CRISPR/Cas9 genome editing in murine zygotes.PLoS One,?13?(5)?:?e0196891.
  • Brinkman EK, Kousholt AN, et al.?(2018)?Easy quantification of template-directed CRISPR/Cas9 editing.?Nucleic Acids Res.?doi: 10.1093/nar/gky164
  • Gregg E. Homanics.?(2018)?Gene edited CRISPy critters for alcohol research.?Alcohol.?doi: 10.1016/j.alcohol.2018.03.001
  • Andersson M, Turesson H, et al.?(2018)?Genome editing in potato via CRISPR-Cas9 ribonucleoprotein delivery.?Physiol Plant.?doi: 10.1111/ppl.12731
  • Ohtsuka M, Sato M, et al.?(2018)?i-GONAD: a robust method for?in situ?germline genome engineering using CRISPR nucleases.?Genome Biol,?19?:?25.
  • Riddle MR, Aspiras AC, et al.?(2018)?Insulin resistance in cavefish as an adaptation to a nutrient-limited environment.?Nature,?555?:?647–651.
  • Seki A, Rutz S.?(2018)?Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells.?J Exp Med.?doi: 10.1084/jem.20171626
  • Han X, Liu Z, et al.?(2017)?Cas9 ribonucleoprotein delivery via microfluidic cell-deformation chip for human T-Cell genome editing and immunotherapy?.?Adv Biosys,?1?:?1600007.
  • Al Abdallah Q, Ge W, Fortwendel JR.?(2017)?A simple and universal system for gene manipulation in?Aspergillus fumigatus:?in vitro-assembled Cas9 guide RNA ribonucleoproteins coupled with microhomology repair templates.?mSphere,?2?:?e00446–17.
  • di Pietro F, Valon L, et al..?(2017)?An RNAi screen in a novel model of oriented divisions identifies the actin-capping protein Z β as an essential regulator of spindle orientation.?Curr Biol,?27?:?2452–2464.
  • Nachmanson D, Lian S, et al.?(2017)?CRISPR-DS: An efficient, low DNA input method for ultra-accurate sequencing.?bioRxiv.?doi: 10.1101/207027
  • Schwinn MK, Machleidt T, et al.?(2017)?CRISPR-mediated tagging of endogenous proteins with a luminescent peptide.?ACS Chem Biol.doi: 10.1021/acschembio.7b00549
  • Xu MM, Pu Y, et al..?(2017)?Dendritic cells but not macrophages sense tumor mitochondrial DNA for cross-priming through signal regulatory protein α signaling.?Immunity,?47?:?363–37.
  • Mikheikin A, Olsen A, et al.?(2017)?DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle.?Nat Commun,?8?:?1665.
  • Quadros RM, Miura H, et al.?(2017)?Easi-CRISPR: a robust method for one-step generation of mice carrying conditional and insertion alleles using long ssDNA donors and CRISPR ribonucleoproteins?.?Genome Biology,?18?:?92.
  • Wefers B, Bashir S, et al.?(2017)?Gene editing in mouse zygotes using the CRISPR/Cas9 system.?Methods,?121–122?:?55–67.
  • Rivera-Torres N, Banas K, et al..?(2017)?Insertional mutagenesis by CRISPR/Cas9 ribonucleoprotein gene editing in cells targeted for point mutation repair directed by short single-stranded DNA oligonucleotides?.?PLoS One,?12?:?e0169350.
  • Agudelo D, Duringer A, et al..?(2017)?Marker-free coselection for CRISPR-driven genome editing in human cells.?Nature Methods,?14?:615–620.
  • Luo L, Bokil N, et al..?(2017)?SCIMP is a transmembrane non-TIR TLR adaptor that promotes proinflammatory cytokine production from macrophages?.?Nat Commun,?8?:?14133.
  • Cuellar TL, Herzner AM, et al.?(2017)?Silencing of retrotransposons by SETDB1 inhibits the interferon response in acute myeloid leukemia.?J Cell Biol,?216?:?3535–3549.
  • Jacobi AM, Rettig GR, Turk R, Collingwood MA, Zeiner SA, Quadros RM, Harms DW, Bonthuis PJ, Gregg C, Ohtsuka M, Gurumurthy CB,?Behlke MA.?(2017)?Simplified CRISPR tools for efficient genome editing and streamlined protocols for their delivery into mammalian cells and mouse zygotes.?Methods,?121–122?:?16–28.
  • Kohler S, Wojcik M, et al..?(2017)?Superresolution microscopy reveals the three-dimensional organization of meiotic chromosome axes in intact?Caenorhabditis elegans?tissue?.?Proc Natl Acad Sci USA,?114?:?E4734–E4743.
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